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Ch 45 Body Fluid Regulation and Excretion, Part II
Davison BI 102 Lecture Notes 2/20 & 2/25 ‘03

Urinary Systems in Humans 

Gross Anatomy of the Human Urinary System (See fig. 45.5) 

Urethra carries urine from the bladder out of the body

Urinary bladder stores urine

A ureter from each kidney fills the bladder with urine.

The expanded end of the ureter at the kidney is called the renal pelvis


Gross Anatomy of the Kidney (see fig. 45.6)

Renal pelvis,  renal medulla and renal cortex.

 

Microanatomy of the kidney (see figs. 45.7, 45.8, 45.9)

Nephrons, collecting ducts, and associated capillary networks (glomerulus and peritubular capillaries

Nephron structure

            Glomerular capsule, proximal tubule, loop w/descending and ascending limb, distal tubule.  A single layer of cuboidal epithelial cells comprises the walls of the nephron.  Microvilli are present on these cells enhancing their transport role by providing more membrane surface to conduct transport (see fig. 45.8).   The nephron is composed of transport epitheilium.

 Collecting duct – larger tube that drains several nephrons output into the renal pelvis.

 Capillary networks

            Glomerulus -  a capillary network surrounding by the glomerular capsule of the nephron.

            Peritubular capillaries – capillary network surrounding the nephron 

Physiological Processes within the Kidney

The Nephron and capillary networks work together in performing three basic processes aimed at two basic, overall kidney functions, molecular waste elimination and maintaining water balance. 

  1. Filtration – occurs in the glomerular capsule.  Much of the blood plasma (water, urea, sugar, etc. –all molecules whether waste or needed nutrients except for the larger blood proteins such as clotting factors and albumin) is forced by blood pressure out of the glomerulus and into the nephron.  On a per day basis, about 180 liters (50 gal.) of filtrate is produced.  Thus, the total blood plasma volume is filtered through nephrons a total of 70 times per day!
  1. Tubular Reabsorptionwater and nutrients are reabsorbed (by the nephron tubules) and reenter the peritubular capillaries.  Needed nutrients that were filtered out are returned to the blood stream by the proximal tubule.  Plasma proteins actively transport these molecules out of the lumen of the nephron and into the lumen of the peritubular capillaries.  As these solutes are taken out of the nephron and placed into the capillaries, water follows passively via osmosis; i.e., water also leaves the nephron and enters the capillaries.  Most (60-70%) of the filtrate is reabsorbed by the proximal tubule and returned to the capillaries that surround the proximal tubule.
  1. Tubular Secretion – waste molecules not filtered out of the blood by the glomerulus may be actively secreted into the nephron along the distal tubule (e.g. ammonia, uric acid, drugs).

Urine is formed by the actions of filtration, tubular secretion, and reabsorption.  The final form of urine takes shape after the fluid from the nephrons has drained through the collecting duct into the renal pelvis.

  

Urine formation and Conservation of Water Require Additional Reabsorption 

Normal urine is a sterile fluid containing water, urea, salt (NaCl), ammonia, and other metabolic wastes.  Some peculiar contents found in urine depend on the chemical nature of what has been ingested (red beets & asparagus stories). 

The amount of water in urine determines the urine’s concentration.  A concentrated urine of small volume containing little water is formed when the body is conserving water.  A dilute urine of larger volume is formed when the body is removing excess water (as when happens after drinking lots of water).  The final concentration of urine (and thus the amount of urine) forms as the tubular fluid flows through the collecting duct into the renal pelvis. 

A key to understanding additional water reabsorption is to know that the inner medulla of the kidney has a very high solute concentration (it is very hypertonic compared to normal body fluids).  Each time the loop of the nephron and the collecting ducts pass down through the inner medulla water is squeezed out of their tubules and reenters the bloodstream.  Consider this analogy:  its like ringing out a dishrag full of water in which you twist the rag once squeezing out water and then relaxing the rag so as to grip it again in preparation for another, harder twist removing even more water.  The first twist of the wet rag represents the descending limb of the loop of the nephron, the period or re-gripping represents the ascending limb of the loop of the nephron (which is impermeable to water movement), and the second twist represents the passage through the collecting duct.  In order to get another grip to squeeze out more water, NaCl is reabsorbed in the ascending loop of the nephron (see below for explanation). 

 

Obligatory Reabsorption of Water 

The descending limb of the loop of the nephron will always reabsorb water as this portion of the nephron is always permeable to water.  The high solute concentration of the inner medulla into which the descending loop penetrates will always draw water out of the renal tubule.  (The dishrag will always get one good squeeze.)  In fact, about 15-25% of the original filtrate volume is reabsorbed in the descending limb of the loop of the nephron.  At the tip of the loop of the nephron, the fluid within the nephron is highly concentrated, yet it still contains salts that need to be reabsorbed and much of the water it contains must also be reabsorbed.   So… 

 

Removal of NaCl from the Ascending Limb of the Nephron Loop 

Before any additional water can be reabsorbed from the fluid inside the nephron , NaCl must be reabsorbed.   The reabsorption of NaCl occurs along the ascending loop of the nephron.  The removal of NaCl from the nephron has the effect of making the nephron fluid dilute without adding water.  A dilute fluid can be passed once again through the inner medulla to force the reabsorption of more water.  This second passage occurs as fluid from the nephron enters the collecting duct and the collecting duct penetrates through the renal medulla to the renal pelvis. 

Variable Reabsorption of Water by the Collecting Duct 

Having passed through the loop of the nephron, only 15% of the original filtrate volume remains in the neprhon tubule to drain to the collecting duct.  This may not sound like much fluid, but on a daily basis this 15% is about 27 liters! (The total volume of blood is only 5-6 liters).  Obviously we don’t urinate 27 liters per day.  Collecting ducts reabsorb much of this volume except for a good liter or so that is eliminated as urine in the case of a healthy adult.  

As the amount of water in body fluids is maintained within narrow limits, a variable amount of water reabsorption occurs in the collecting ducts depending on the body’s need to conserve water or eliminate excess water (produce a concentrated urine or produce a dilute urine).  Whether one produces concentrated or dilute urine depends upon several factors:  1) dietary intake of water, 2) water loss through perspiration or diarrhea, and 3) a hormonal signal from the brain that regulates variable water reabsorption by the collecting ducts.  Based on the water level detected in body fluids passing through the brain, variable amounts of antidiuretic hormone (ADH) are released from the pituitary gland.  Since “diuresis” basically means “more urine,” antidiuretic hormone will promote less urine formation.  The volume of urine can be less while the amount of metabolic waste removed will be unaltered.  

Antidiuretic hormone released from the brain (pituitary) travels to the kidneys and enters the filtrate.  ADH has does not have any effect until it reaches the walls of the collecting duct.  There ADH stimulates the opening of water channels which, when opened, speed the rate of water movement via osmosis out of the collecting duct.  Simply stated, ADH promotes water reabsorption by the collecting duct.  Should the body not need as much water reabsorbed, the brain will not be sending as much ADH into the bloodstream.  A dilute urine would result.  Should water need conserving, more ADH is released and a concentrated urine would result. 

A dilute urine may form under the influence of alcohol which suppresses the release of antidiuretic hormone.  Even though your brain senses that water content in body fluids should be conserved, the hormonal signal ADH isn’t released in times of drunkenness.  The result is copious urination that removes precious water from the body.  A hangover is, at least in part, due to the dehydration that results.